Hotspot

trnT-UGU-trnL-UAA

LSC · 49160-50356 · 1197 bp

Back to Cotoneaster

Pi 0.00636

MarkerSeek score 51.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1197
variable_sites: 39
pi: 0.00636
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	1026-1343 bp	0.985075	84.8
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	1034-1351 bp	0.985075	84.2
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	1058-1375 bp	0.985075	83.8
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	1066-1383 bp	0.985075	83.3
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	1040-1357 bp	0.985075	82.9