Hotspot

petN-psbM

LSC · 30062-31263 · 1202 bp

Back to Cotoneaster

Pi 0.0023

MarkerSeek score 52.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1202
variable_sites: 30
pi: 0.0023
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	50.0	45.0	58.0	57.6	1285-1392 bp	0.985075	81.5
petN-psbM_p2	AGTATGGGGAAGGAGTGGAC	AAAACAGTCAGCCAAGGTGA	55.0	45.0	58.1	57.6	1228-1335 bp	0.985075	80.9
petN-psbM_p3	GTATGGGGAAGGAGTGGACT	AAAACAGTCAGCCAAGGTGA	55.0	45.0	58.1	57.6	1227-1334 bp	0.985075	80.9
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	AAAACAGTCAGCCAAGGTGA	55.0	45.0	57.8	57.6	1226-1333 bp	0.985075	80.4
petN-psbM_p5	GGGGAAGGAGTGGACTCTAG	AAAACAGTCAGCCAAGGTGA	60.0	45.0	58.2	57.6	1223-1330 bp	0.985075	80.1