Hotspot

rps19

LSC · 87114-87263 · 150 bp

Back to Cotinus

Pi 0.056

MarkerSeek score 67.20

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 150
variable_sites: 24
pi: 0.056
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	CTCATAAGAACGCCCACGAA	CCGGATCGAAATGTTGGCTA	50.0	50.0	58.0	58.1	526-993 bp	1.0	90.9
rps19_p2	TAAGAACGCCCACGAATCTG	CCGGATCGAAATGTTGGCTA	50.0	50.0	58.0	58.1	522-989 bp	1.0	90.9
rps19_p3	CTCATAAGAACGCCCACGAA	TAGTTATGGGCTATGGGCGA	50.0	50.0	58.0	58.0	434-625 bp	0.857143	86.1
rps19_p4	TAAGAACGCCCACGAATCTG	TAGTTATGGGCTATGGGCGA	50.0	50.0	58.0	58.0	430-621 bp	0.857143	86.1
rps19_p5	TTTGGCTGTGCAAACCAAAG	TAGTTATGGGCTATGGGCGA	45.0	50.0	58.0	58.0	867-1064 bp	0.857143	86.0