Hotspot

rps19-rpl2

LSC · 87264-87445 · 182 bp

Back to Cotinus

Pi 0.0957
MarkerSeek score 80.30
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
LSC
length_bp
182
variable_sites
34
pi
0.0957
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 ATAGCAATGGTATGGCCGAC CTTCGCCGCCGTAGTAAATA 50.0 50.0 57.8 57.9 226-516 bp 0.714286 76.6
rps19-rpl2_p2 ATAGCAATGGTATGGCCGAC TTCGCCGCCGTAGTAAATAG 50.0 50.0 57.8 57.9 225-515 bp 0.714286 76.6
rps19-rpl2_p3 ATAGCAATGGTATGGCCGAC TAGAAAAAGACCCGCAACCC 50.0 50.0 57.8 58.1 311-601 bp 0.714286 74.7
rps19-rpl2_p4 ATAGCAATGGTATGGCCGAC TTGGGGTTATCCTGCACTTG 50.0 50.0 57.8 57.8 290-580 bp 0.714286 74.3
rps19-rpl2_p5 ATAGGCAAATGCTCCCTTCC TAGAAAAAGACCCGCAACCC 50.0 50.0 57.6 58.1 338-628 bp 0.714286 72.8