Hotspot

atpH-atpI

LSC · 15141-16194 · 1054 bp

Back to Cotinus

Pi 0.00345

MarkerSeek score 62.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1054
variable_sites: 12
pi: 0.00345
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TACAAGCGGGATTCAAGCTC	50.0	50.0	58.0	58.0	1195-1199 bp	1.0	80.6
atpH-atpI_p2	TACCTTCTACAGCTTGGCCT	TACAAGCGGGATTCAAGCTC	50.0	50.0	58.0	58.0	1244-1248 bp	1.0	80.5
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.1	58.0	1159-1163 bp	1.0	80.1
atpH-atpI_p4	AATAGAAGCAAGCCCGACAG	CCGGTCATGTTCCTTGGATT	50.0	50.0	58.0	57.9	1218-1222 bp	1.0	79.8
atpH-atpI_p5	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1171-1175 bp	1.0	79.8