Hotspot

trnS-GCU-trnG-UCC

LSC · 9763-10516 · 754 bp

Back to Cotinus

Pi 0.00777
MarkerSeek score 60.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
754
variable_sites
19
pi
0.00777
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA ACGAATCGCACTTTTACCACT 45.0 42.9 58.0 58.2 798-831 bp 1.0 75.5
trnS-GCU-trnG-UCC_p2 TAGCAATCCGCCGCTTTAAT ACGAATCGCACTTTTACCACT 45.0 42.9 58.0 58.2 796-829 bp 1.0 75.5
trnS-GCU-trnG-UCC_p3 ATTAGCAATCCGCCGCTTTA ACGAATCGCACTTTTACCAC 45.0 45.0 58.0 56.8 798-831 bp 1.0 75.5
trnS-GCU-trnG-UCC_p4 TAGCAATCCGCCGCTTTAAT ACGAATCGCACTTTTACCAC 45.0 45.0 58.0 56.8 796-829 bp 1.0 75.5
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.2 58.2 847-880 bp 1.0 74.3