Hotspot

psbK-psbI

LSC · 9015-9397 · 383 bp

Back to Cotinus

Pi 0.0121

MarkerSeek score 60.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 383
variable_sites: 16
pi: 0.0121
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	512-520 bp	1.0	76.0
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	509-517 bp	1.0	74.0
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	510-518 bp	1.0	74.0
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	511-519 bp	1.0	74.0
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGTCCAGGATTACG	50.0	47.6	57.9	57.8	513-521 bp	1.0	73.6