Hotspot

rpl16

LSC · 89050-91365 · 2316 bp

Pi 0.0102

MarkerSeek score 56.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 2316
variable_sites: 39
pi: 0.0102
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCGGTTGTTTTTCATCCTCG	CGATTGACCATTTCTTAGAATTGC	50.0	37.5	58.0	57.0	2301-2370 bp	1.0	45.3
rpl16_p2	GCGGTTGTTTTTCATCCTCG	CGATTGACCATTTCTTAGAATTGCT	50.0	36.0	58.0	58.2	2301-2370 bp	1.0	45.3
rpl16_p3	GCGGTTGTTTTTCATCCTCG	TCGATTGACCATTTCTTAGAATTGC	50.0	36.0	58.0	58.2	2302-2371 bp	1.0	45.3
rpl16_p4	GCGGTTGTTTTTCATCCTCG	TCTTTTTCCGTTAAATCAACCA	50.0	31.8	58.0	54.5	2370-2439 bp	1.0	45.3
rpl16_p5	GCGGTTGTTTTTCATCCTCG	CCGTTAAATCAACCAAAAATAAGCA	50.0	32.0	58.0	57.4	2363-2432 bp	1.0	45.3