Hotspot

ndhF

SSC · 121170-123404 · 2235 bp

Pi 0.00179

MarkerSeek score 24.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 6
pi: 0.00179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCAGTGAGGAGAAAACCGGA	AAGTTCAGAGTGTCATGCCC	50.0	50.0	58.3	57.8	2858-2884 bp	1.0	76.6
ndhF_p2	TCAGTGAGGAGAAAACCGGA	CAGAGTGTCATGCCCGTAAT	50.0	50.0	58.3	57.7	2853-2879 bp	1.0	75.6
ndhF_p3	CAGTGAGGAGAAAACCGGAA	AAGTTCAGAGTGTCATGCCC	50.0	50.0	57.5	57.8	2857-2883 bp	1.0	74.6
ndhF_p4	ATGCGTGCTCAAAGACGTAA	CAGAGTGTCATGCCCGTAAT	45.0	50.0	58.2	57.7	2997 bp	0.5	55.8
ndhF_p5	TCTATGCGTGCTCAAAGACG	CAGAGTGTCATGCCCGTAAT	50.0	50.0	58.4	57.7	3000 bp	0.5	54.5