Hotspot

rps15-ycf1

SSC · 135879-136515 · 637 bp

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Pi 0.00733
MarkerSeek score 45.70
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
637
variable_sites
7
pi
0.00733
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCAACAGAACCCTTGTTTTCT GCTTGTCTGAATCGCTATTGG 38.1 47.6 55.5 57.4 801-844 bp 1.0 41.9
rps15-ycf1_p2 TCAACAGAACCCTTGTTTTCT CTTTGGCCCAATTACCGATT 38.1 45.0 55.5 56.4 831-874 bp 1.0 41.9
rps15-ycf1_p3 TCAACAGAACCCTTGTTTTCT TGGCCCAATTACCGATTAGA 38.1 45.0 55.5 56.3 828-871 bp 1.0 41.9
rps15-ycf1_p4 TCAACAGAACCCTTGTTTTCTTC GCTTGTCTGAATCGCTATTGG 39.1 47.6 57.4 57.4 801-844 bp 1.0 41.9
rps15-ycf1_p5 CAACAGAACCCTTGTTTTCTTCT GCTTGTCTGAATCGCTATTGG 39.1 47.6 57.4 57.4 800-843 bp 1.0 41.9