Hotspot

psaC-ndhE

SSC · 128995-130071 · 1077 bp

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Pi 0.00498
MarkerSeek score 49.10
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
1077
variable_sites
8
pi
0.00498
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.91
markerseek_score
49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ATACATCTGTAGGGCAGGCT CTGAAGCAGCTATTGGGCTA 50.0 50.0 57.9 57.4 1189-1245 bp 1.0 78.6
psaC-ndhE_p2 ATACATCTGTAGGGCAGGCT TGAAGCAGCTATTGGGCTAG 50.0 50.0 57.9 57.4 1188-1244 bp 1.0 78.6
psaC-ndhE_p3 ACATCCGTCCCAAGGTATCA CTGAAGCAGCTATTGGGCTA 50.0 50.0 58.1 57.4 1215-1271 bp 1.0 78.5
psaC-ndhE_p4 ACATCCGTCCCAAGGTATCA TGAAGCAGCTATTGGGCTAG 50.0 50.0 58.1 57.4 1214-1270 bp 1.0 78.5
psaC-ndhE_p5 ATACATCTGTAGGGCAGGCT GAAGCAGCTATTGGGCTAGC 50.0 55.0 57.9 58.8 1187-1243 bp 1.0 77.5