Hotspot

ndhF-rpl32

SSC · 123405-124321 · 917 bp

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Pi 0
MarkerSeek score 44.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
917
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCTGTCGGAATCGGAAT GAAAAAGCCTTTACTGCGGC 50.0 50.0 58.6 58.3 1076-1119 bp 1.0 75.7
ndhF-rpl32_p2 TTTTGTTGCTGTCGGAATCG GAAAAAGCCTTTACTGCGGC 45.0 50.0 57.3 58.3 1080-1123 bp 1.0 75.3
ndhF-rpl32_p3 TGCTGTCGGAATCGGAATAA GAAAAAGCCTTTACTGCGGC 45.0 50.0 57.0 58.3 1074-1117 bp 1.0 73.0
ndhF-rpl32_p4 TTGCTGTCGGAATCGGAATA GAAAAAGCCTTTACTGCGGC 45.0 50.0 57.0 58.3 1075-1118 bp 1.0 73.0
ndhF-rpl32_p5 TTTTTGTTGCTGTCGGAATCG GAAAAAGCCTTTACTGCGGC 42.9 50.0 58.0 58.3 1081-1124 bp 1.0 72.7