Hotspot

rpl20-rps12

LSC · 76405-77166 · 762 bp

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Pi 0.00175
MarkerSeek score 44.90
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
762
variable_sites
2
pi
0.00175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 AGTCTTGAATGAGCTCCCCT TCTCCCGCTCTTAGAGGATG 50.0 55.0 58.0 58.0 885-908 bp 1.0 82.5
rpl20-rps12_p2 TGAGCTCCCCTAAAGGTTGA TCTCCCGCTCTTAGAGGATG 50.0 55.0 58.0 58.0 876-899 bp 1.0 82.5
rpl20-rps12_p3 TTTTTGTTCGACGTCTCCGA TCTCCCGCTCTTAGAGGATG 45.0 55.0 57.8 58.0 842-865 bp 1.0 81.3
rpl20-rps12_p4 TCTCCGAGCTGTATATCCCC TCTCCCGCTCTTAGAGGATG 55.0 55.0 57.8 58.0 829-852 bp 1.0 81.2
rpl20-rps12_p5 ATCCCCGTCTAAGTCTGGTC TCTCCCGCTCTTAGAGGATG 55.0 55.0 58.2 58.0 815-838 bp 1.0 81.2