Hotspot

accD-psaI

LSC · 65263-66143 · 881 bp

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Pi 0.00227
MarkerSeek score 45.20
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
881
variable_sites
3
pi
0.00227
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CACGGTTTCCTTCCCTTGAA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 969-982 bp 1.0 83.8
accD-psaI_p2 CACGGTTTCCTTCCCTTGAA AGAAACCATTGCAATTGCCG 50.0 45.0 58.0 57.9 974-987 bp 1.0 83.4
accD-psaI_p3 TCAACTCCACGGTTTCCTTC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.8 58.0 976-989 bp 1.0 82.0
accD-psaI_p4 TCAACTCCACGGTTTCCTTC AGAAACCATTGCAATTGCCG 50.0 45.0 57.8 57.9 981-994 bp 1.0 81.6
accD-psaI_p5 TTCAACTCCACGGTTTCCTT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.3 58.0 977-990 bp 1.0 78.3