Hotspot

ndhC-trnV-UAC

LSC · 55655-57669 · 2015 bp

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Pi 0.00496
MarkerSeek score 42.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
2015
variable_sites
15
pi
0.00496
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 2150-2152 bp 1.0 79.3
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 2144-2146 bp 1.0 78.5
ndhC-trnV-UAC_p3 CTAACCGGGGCTAAAACTCC AGCCCAATGCAACAAAATGC 55.0 45.0 58.0 58.5 2232-2234 bp 1.0 77.1
ndhC-trnV-UAC_p4 CTAACCGGGGCTAAAACTCC GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.0 57.5 2149-2151 bp 1.0 76.6
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAT AGCCCAATGCAACAAAATGC 50.0 45.0 57.9 58.5 2226-2228 bp 1.0 76.2