Hotspot

rps4-trnT-UGU

LSC · 50635-51133 · 499 bp

Back to Costus

Pi 0.012
MarkerSeek score 49.60
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
499
variable_sites
9
pi
0.012
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TAGTCCTGGTAAAACCCCCA ATTATGTGAGCCCGCTTAGC 50.0 50.0 57.6 58.1 645-651 bp 1.0 79.3
rps4-trnT-UGU_p2 TAGTCCTGGTAAAACCCCCA CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.6 57.9 626-632 bp 1.0 79.3
rps4-trnT-UGU_p3 TAGTCCTGGTAAAACCCCCA GATGGTCATCGGTTCGACTC 50.0 55.0 57.6 58.2 595-601 bp 1.0 79.2
rps4-trnT-UGU_p4 TAGTCCTGGTAAAACCCCCA GTGATTATGTGAGCCCGCTT 50.0 50.0 57.6 58.3 648-654 bp 1.0 77.5
rps4-trnT-UGU_p5 TAGTCCTGGTAAAACCCCCA ATGTGATTATGTGAGCCCGC 50.0 50.0 57.6 58.4 650-656 bp 1.0 77.0