Hotspot

trnS-GCU-trnG-UCC

LSC · 9417-11388 · 1972 bp

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Pi 0.0112
MarkerSeek score 69.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1972
variable_sites
36
pi
0.0112
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 TTAGCAATCCGACGCCTTAG AACTGAGAGACCCCTTAACT 50.0 45.0 57.8 55.1 1999-2083 bp 1.0 60.2
trnS-GCU-trnG-UCC_p2 TTAGCAATCCGACGCCTTAG ACTGAGAGACCCCTTAACTT 50.0 45.0 57.8 55.1 1998-2082 bp 1.0 60.2
trnS-GCU-trnG-UCC_p3 TTAGCAATCCGACGCCTTAG ACTGAGAGACCCCTTAACTTTTG 50.0 43.5 57.8 58.0 1998-2082 bp 1.0 59.5
trnS-GCU-trnG-UCC_p4 CGGAGAGAGAGGGATTCGAA AACTGAGAGACCCCTTAACT 55.0 45.0 58.3 55.1 2048-2132 bp 1.0 59.4
trnS-GCU-trnG-UCC_p5 CGGAGAGAGAGGGATTCGAA ACTGAGAGACCCCTTAACTT 55.0 45.0 58.3 55.1 2047-2131 bp 1.0 59.4