Hotspot

syfB

SSC · 26914-29031 · 2118 bp

Back to Coscinodiscus

Pi 0.375

MarkerSeek score 71.90

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: SSC
length_bp: 2118
variable_sites: 491
pi: 0.375
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.54
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	TTTAATAAGGGCGAGCGACC	CTTTGACTCCGCCATTCGTA	50.0	50.0	58.1	58.0	2996 bp	0.333333	52.8
syfB_p2	TTTAATAAGGGCGAGCGACC	TTTGACTCCGCCATTCGTAG	50.0	50.0	58.1	58.0	2995 bp	0.333333	52.8
syfB_p3	TTTAATAAGGGCGAGCGACC	AAGACGGTAGCACAGGTACT	50.0	50.0	58.1	58.1	2831 bp	0.333333	52.3
syfB_p4	TTTAATAAGGGCGAGCGACC	CAAAAGACGGTAGCACAGGT	50.0	50.0	58.1	58.1	2834 bp	0.333333	51.9
syfB_p5	TTTAATAAGGGCGAGCGACC	GGTCAAAAGACGGTAGCACA	50.0	50.0	58.1	58.1	2837 bp	0.333333	51.9