Hotspot

rrn5

IRb · 15085-15402 · 318 bp

Back to Coscinodiscus

Pi 0.398

MarkerSeek score 66.10

Primer available yes

Label rrn5

Feature Metadata

label_name: rrn5
feature_type: rRNA
region: IRb
length_bp: 318
variable_sites: 59
pi: 0.398
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.46
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5_p1	ATCTCCCCCAAGAGTTCACA	GTAGCTGCACACGGTTACTT	50.0	50.0	58.0	58.2	1357-1655 bp	0.666667	76.6
rrn5_p2	TCTCCCCCAAGAGTTCACAT	GTAGCTGCACACGGTTACTT	50.0	50.0	58.0	58.2	1356-1654 bp	0.666667	76.6
rrn5_p3	TAGTAAACGGAACCACGCTG	GTAGCTGCACACGGTTACTT	50.0	50.0	57.9	58.2	852-1150 bp	0.666667	76.5
rrn5_p4	GTAGTAAACGGAACCACGCT	GTAGCTGCACACGGTTACTT	50.0	50.0	57.9	58.2	853-1151 bp	0.666667	76.4
rrn5_p5	TTCGGTCCATATCCGGTGTA	GTAGCTGCACACGGTTACTT	50.0	50.0	57.9	58.2	1212-1510 bp	0.666667	76.4