Hotspot

rpoC2

LSC · 105738-109985 · 4248 bp

Back to Coscinodiscus

Pi 0.206

MarkerSeek score 73.70

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4248
variable_sites: 875
pi: 0.206
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GGCTTGAAGAAGAAACTCACC	GCATCAGACGAGGTATTGAGA	47.6	47.6	57.1	57.3	1143 bp	0.333333	24.3
rpoC2_p2	GGCTTGAAGAAGAAACTCACC	AGCATCAGACGAGGTATTGAG	47.6	47.6	57.1	57.3	1144 bp	0.333333	24.3
rpoC2_p3	GGCTTGAAGAAGAAACTCACC	ACGAGAAGGAATCCATCGAT	47.6	45.0	57.1	56.1	1007 bp	0.333333	23.1
rpoC2_p4	GGCTTGAAGAAGAAACTCACC	AGCATCAGACGAGGTATTGA	47.6	45.0	57.1	56.1	1144 bp	0.333333	22.6
rpoC2_p5	GGCTTGAAGAAGAAACTCACC	AGGTTGACCCTTTTGTAGTGT	47.6	42.9	57.1	57.1	932 bp	0.333333	22.5