Hotspot

rpl2

SSC · 35116-35943 · 828 bp

Back to Coscinodiscus

Pi 0.196

MarkerSeek score 79.60

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: SSC
length_bp: 828
variable_sites: 227
pi: 0.196
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	AAAGGTGGGGGCGTTATTTT	CAATGTTAATGGGCCTTGCG	45.0	50.0	58.0	58.1	1925 bp	0.333333	52.6
rpl2_p2	AAGGTGGGGGCGTTATTTTT	CAATGTTAATGGGCCTTGCG	45.0	50.0	58.0	58.1	1924 bp	0.333333	52.6
rpl2_p3	AAAGGTGGGGGCGTTATTTT	ATGTTAATGGGCCTTGCGAA	45.0	45.0	58.0	58.2	1923 bp	0.333333	52.0
rpl2_p4	AAGGTGGGGGCGTTATTTTT	AATGTTAATGGGCCTTGCGA	45.0	45.0	58.0	58.2	1923 bp	0.333333	52.0
rpl2_p5	AAGGTGGGGGCGTTATTTTT	ATGTTAATGGGCCTTGCGAA	45.0	45.0	58.0	58.2	1922 bp	0.333333	52.0