Hotspot

rbcL

LSC · 1-1473 · 1473 bp

Back to Coscinodiscus

Pi 0.0706

MarkerSeek score 79.70

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1473
variable_sites: 153
pi: 0.0706
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	ATTCCAAGGTCCTGCAACAG	GCACCTTCGTTACGAGCTAA	50.0	50.0	58.1	58.0	861 bp	0.333333	52.5
rbcL_p2	ATTCCAAGGTCCTGCAACAG	AACACCAGACATACGCATCC	50.0	50.0	58.1	58.0	526 bp	0.333333	52.5
rbcL_p3	GCTGTTGCTGCTTTACGTTT	GCACCTTCGTTACGAGCTAA	45.0	50.0	57.9	58.0	917 bp	0.333333	52.3
rbcL_p4	GCTGTTGCTGCTTTACGTTT	AACACCAGACATACGCATCC	45.0	50.0	57.9	58.0	582 bp	0.333333	52.2
rbcL_p5	ATTCCAAGGTCCTGCAACAG	TACCAACAACTGTACCAGCG	50.0	50.0	58.1	57.8	557 bp	0.333333	51.4