Hotspot

bas1

IRb · 5739-6350 · 612 bp

Back to Coscinodiscus

Pi 0.14

MarkerSeek score 80.80

Primer available yes

Label bas1

Feature Metadata

label_name: bas1
feature_type: gene
region: IRb
length_bp: 612
variable_sites: 119
pi: 0.14
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
bas1_p1	GTTAGTCGCGGATCAACAGT	GATGACCTTTGAAAGCGTGC	50.0	50.0	58.0	58.0	1054 bp	0.333333	53.0
bas1_p2	ATCAAACGGGAACAGTTGCT	GATGACCTTTGAAAGCGTGC	45.0	50.0	58.0	58.0	1002 bp	0.333333	52.9
bas1_p3	GTTAGTCGCGGATCAACAGT	CCGTTTTCGTGCTACGTTTC	50.0	50.0	58.0	58.1	1199 bp	0.333333	52.7
bas1_p4	GTTAGTCGCGGATCAACAGT	CGTTTTCGTGCTACGTTTCC	50.0	50.0	58.0	58.1	1198 bp	0.333333	52.7
bas1_p5	ATCAAACGGGAACAGTTGCT	CGTTTTCGTGCTACGTTTCC	45.0	50.0	58.0	58.1	1146 bp	0.333333	52.6