Hotspot

acpP-rpl20

IRa · 65381-67050 · 1670 bp

Back to Coscinodiscus

Pi 0.169

MarkerSeek score 81.30

Primer available yes

Label acpP-rpl20

Feature Metadata

label_name: acpP-rpl20
feature_type: igs
region: IRa
length_bp: 1670
variable_sites: 392
pi: 0.169
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
acpP-rpl20_p1	AGGATTTGAACCTACGGCAC	ACAAGCCTGTCCTGTAGAGT	50.0	50.0	57.9	58.0	2818 bp	0.333333	52.3
acpP-rpl20_p2	AGGATTTGAACCTACGGCAC	GCACGCTTTCAAAGGTCATC	50.0	50.0	57.9	58.0	2619 bp	0.333333	52.2
acpP-rpl20_p3	AGGATTTGAACCTACGGCAC	CCTGTCCTGTAGAGTGGGAA	50.0	55.0	57.9	58.1	2813 bp	0.333333	52.0
acpP-rpl20_p4	AGGATTTGAACCTACGGCAC	GAAACGTAGCACGAAAACGG	50.0	50.0	57.9	58.1	2474 bp	0.333333	51.9
acpP-rpl20_p5	AGGATTTGAACCTACGGCAC	GGAAACGTAGCACGAAAACG	50.0	50.0	57.9	58.1	2475 bp	0.333333	51.9