Hotspot

trnI(gau)-rns

IRa · 62177-62250 · 74 bp

Back to Coscinodiscus

Pi 0.234

MarkerSeek score 81.40

Primer available yes

Label trnI(gau)-rns

Feature Metadata

label_name: trnI(gau)-rns
feature_type: igs
region: IRa
length_bp: 74
variable_sites: 23
pi: 0.234
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI(gau)-rns_p1	ATTTGAACCAGAGACCTCGC	GTAACCGTACTGGAAGGTGC	50.0	55.0	57.9	58.3	162-175 bp	1.0	88.9
trnI(gau)-rns_p2	ATTTGAACCAGAGACCTCGC	AGGTAACCGTACTGGAAGGT	50.0	50.0	57.9	57.7	164-177 bp	1.0	88.7
trnI(gau)-rns_p3	CTGGGCCATTCTGGATTTGA	GTAACCGTACTGGAAGGTGC	50.0	55.0	57.9	58.3	176-189 bp	1.0	88.6
trnI(gau)-rns_p4	CTGGGCCATTCTGGATTTGA	AGGTAACCGTACTGGAAGGT	50.0	50.0	57.9	57.7	178-191 bp	1.0	88.3
trnI(gau)-rns_p5	ATTTGAACCAGAGACCTCGC	GGTAACCGTACTGGAAGGTG	50.0	55.0	57.9	57.6	163-176 bp	1.0	88.2