Hotspot

trnL(uaa)-trnC(gca)

SSC · 49963-50030 · 68 bp

Back to Coscinodiscus

Pi 0.468

MarkerSeek score 79.40

Primer available yes

Label trnL(uaa)-trnC(gca)

Feature Metadata

label_name: trnL(uaa)-trnC(gca)
feature_type: igs
region: SSC
length_bp: 68
variable_sites: 42
pi: 0.468
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL(uaa)-trnC(gca)_p1	CTCACGCACTATCACTAGGC	CAGTTCGAATCTGGGTGCC	55.0	57.9	57.9	58.5	151-156 bp	1.0	78.4
trnL(uaa)-trnC(gca)_p2	CTCACGCACTATCACTAGGC	CCAGTTCGAATCTGGGTGC	55.0	57.9	57.9	58.5	152-157 bp	1.0	78.4
trnL(uaa)-trnC(gca)_p3	TAAGTCCGTCGTGTCTACCA	CAGTTCGAATCTGGGTGCC	50.0	57.9	57.8	58.5	122-127 bp	1.0	78.3
trnL(uaa)-trnC(gca)_p4	ACCCTCACGCACTATCACTA	CAGTTCGAATCTGGGTGCC	50.0	57.9	57.9	58.5	154-159 bp	1.0	78.2
trnL(uaa)-trnC(gca)_p5	ACCCTCACGCACTATCACTA	CCAGTTCGAATCTGGGTGC	50.0	57.9	57.9	58.5	155-160 bp	1.0	78.2