Hotspot

ndhE-ndhG

SSC · 118708-118927 · 220 bp

Back to Corynonema

Pi 0.0463

MarkerSeek score 79.70

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 220
variable_sites: 10
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AGTTCAAGACACATCAGGGC	TTGATAGGTGCAATTGCCGT	50.0	45.0	57.8	58.2	360-392 bp	1.0	80.1
ndhE-ndhG_p2	CAGGGCCCGAACCATATTTC	TTGATAGGTGCAATTGCCGT	55.0	45.0	58.7	58.2	346-378 bp	1.0	76.3
ndhE-ndhG_p3	GTTCAAGACACATCAGGGCC	TTGATAGGTGCAATTGCCGT	55.0	45.0	58.8	58.2	359-391 bp	1.0	75.0
ndhE-ndhG_p4	ATCAGGGCCCGAACCATATT	TTGATAGGTGCAATTGCCGT	50.0	45.0	58.9	58.2	348-380 bp	1.0	74.9
ndhE-ndhG_p5	AGTTCAAGACACATCAGGGC	TGATAGGTGCAATTGCCGTG	50.0	50.0	57.8	58.9	359-391 bp	1.0	74.2