Hotspot

psaC-ndhE

SSC · 118162-118401 · 240 bp

Back to Corynonema

Pi 0.05

MarkerSeek score 75.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 240
variable_sites: 12
pi: 0.05
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TGCTTTACATCCGTCCCAAG	AGCAGCTATCGGATCAGCTA	50.0	50.0	57.9	58.1	436 bp	1.0	80.7
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	AGCAGCTATCGGATCAGCTA	50.0	50.0	58.1	58.1	430 bp	1.0	80.6
psaC-ndhE_p3	CAAGCTCGGACACATTGAGT	AGCAGCTATCGGATCAGCTA	50.0	50.0	58.2	58.1	390 bp	1.0	80.2
psaC-ndhE_p4	AATACATCTGTGGGGCAAGC	AGCAGCTATCGGATCAGCTA	50.0	50.0	58.2	58.1	405 bp	1.0	79.8
psaC-ndhE_p5	CGGACACATTGAGTACACCC	AGCAGCTATCGGATCAGCTA	55.0	50.0	58.3	58.1	384 bp	1.0	79.6