Hotspot

rpl32-trnL-UAG

SSC · 114211-114816 · 606 bp

Back to Corynonema

Pi 0.0475
MarkerSeek score 75.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
606
variable_sites
26
pi
0.0475
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAAATCATAGGCCGGTCCAG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1076 bp 0.5 59.0
rpl32-trnL-UAG_p2 GAAATCATAGGCCGGTCCAG CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1077 bp 0.5 59.0
rpl32-trnL-UAG_p3 CCAGAGCGTTCCCCAATAAA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1060 bp 0.5 59.0
rpl32-trnL-UAG_p4 CCAGAGCGTTCCCCAATAAA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 1061 bp 0.5 58.9
rpl32-trnL-UAG_p5 GAAATCATAGGCCGGTCCAG CGAAGCAAAACGGCTCATTT 55.0 45.0 58.1 57.9 1787 bp 0.5 58.7