Hotspot

rpl36-rps8

LSC · 80482-80837 · 356 bp

Back to Corynonema

Pi 0.0408

MarkerSeek score 73.10

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 14
pi: 0.0408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TGCGGATCAGTCGACATTTT	GCGGCTCGACTAGAAAGAAT	45.0	50.0	57.9	57.8	454-462 bp	1.0	82.3
rpl36-rps8_p2	CCGAACGGAAGCCCTTATTT	GCGGCTCGACTAGAAAGAAT	50.0	50.0	58.3	57.8	423-431 bp	1.0	81.3
rpl36-rps8_p3	TGCGGATCAGTCGACATTTT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.9	58.4	435-443 bp	1.0	81.0
rpl36-rps8_p4	TGCGGATCAGTCGACATTTT	CGGCGGAGAAATTTTGTGTT	45.0	45.0	57.9	57.6	434-442 bp	1.0	80.9
rpl36-rps8_p5	TGCGGATCAGTCGACATTTT	CGGGGTATAATGACAGACCG	45.0	55.0	57.9	57.6	475-483 bp	1.0	80.3