Hotspot

petA-psbJ

LSC · 63503-64573 · 1071 bp

Back to Corynonema

Pi 0.0495
MarkerSeek score 74.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1071
variable_sites
53
pi
0.0495
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GTCCAATTGTCCGAAATGAA CCGGATTAGGTTCGTCCTTG 40.0 55.0 54.2 58.1 1121-1193 bp 1.0 52.7
petA-psbJ_p2 GTCCAATTGTCCGAAATGAA TCCGGATTAGGTTCGTCCTT 40.0 50.0 54.2 58.1 1122-1194 bp 1.0 52.1
petA-psbJ_p3 GTCCAATTGTCCGAAATGAAT CCGGATTAGGTTCGTCCTTG 38.1 55.0 54.8 58.1 1121-1193 bp 1.0 49.7
petA-psbJ_p4 GTCCAATTGTCCGAAATGAAT TCCGGATTAGGTTCGTCCTT 38.1 50.0 54.8 58.1 1122-1194 bp 1.0 49.1
petA-psbJ_p5 GTCCAATTGTCCGAAATGAATT CCGGATTAGGTTCGTCCTTG 36.4 55.0 55.6 58.1 1121-1193 bp 1.0 48.0