Hotspot

trnL-UAA-trnF-GAA

LSC · 49625-49988 · 364 bp

Back to Corynonema

Pi 0.047

MarkerSeek score 72.80

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 364
variable_sites: 17
pi: 0.047
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	418-420 bp	1.0	79.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	454-456 bp	1.0	79.1
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	430-432 bp	1.0	78.6
trnL-UAA-trnF-GAA_p4	TTCAAGTCCCTCTATCCCCA	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.0	58.0	407-409 bp	1.0	76.0
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	451-453 bp	1.0	75.9