Hotspot

trnS-GGA-rps4

LSC · 46896-47170 · 275 bp

Back to Corynonema

Pi 0.0595
MarkerSeek score 69.40
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
275
variable_sites
16
pi
0.0595
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 GGGTTCGAATCCCTCTCTTT TGACTCTTCACGCATTCCAA 50.0 45.0 56.9 57.5 414-433 bp 1.0 71.3
trnS-GGA-rps4_p2 GGGTTCGAATCCCTCTCTTT TTGACTCTTCACGCATTCCA 50.0 45.0 56.9 57.5 415-434 bp 1.0 71.3
trnS-GGA-rps4_p3 TTCGAATCCCTCTCTTTCCG TGACTCTTCACGCATTCCAA 50.0 45.0 57.0 57.5 411 bp 0.5 48.0
trnS-GGA-rps4_p4 TTCGAATCCCTCTCTTTCCG TTGACTCTTCACGCATTCCA 50.0 45.0 57.0 57.5 412 bp 0.5 48.0
trnS-GGA-rps4_p5 TTCGAATCCCTCTCTTTCCG AGTCAATGGGTCGGTTTGAA 50.0 45.0 57.0 57.4 361 bp 0.5 47.1