Hotspot

atpF-atpH

LSC · 13303-13776 · 474 bp

Back to Corynonema

Pi 0.0486

MarkerSeek score 70.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 474
variable_sites: 23
pi: 0.0486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGGTCCAGCCTATTCTTGA	GTTGTGGCATTAGCGCTTTT	50.0	45.0	58.0	57.9	697-701 bp	1.0	82.2
atpF-atpH_p2	TGGGTCCAGCCTATTCTTGA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	889-893 bp	1.0	81.9
atpF-atpH_p3	GGTGCTTTGTCTGGACTTCA	GTTGTGGCATTAGCGCTTTT	50.0	45.0	58.0	57.9	910-915 bp	1.0	81.9
atpF-atpH_p4	GGTGCTTTGTCTGGACTTCA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	1102-1107 bp	1.0	81.8
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	57.9	58.0	777-781 bp	1.0	81.6