Hotspot

ycf1

SSC · 129015-134684 · 5670 bp

Back to Corymbia

Pi 0.00366

MarkerSeek score 44.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5670
variable_sites: 52
pi: 0.00366
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGATCCTTTATTTAGAGCTTTCA	ACATGTCGAAACAATGGAAAAGA	33.3	34.8	55.8	57.2	217 bp	1.0	42.6
ycf1_p2	TCCTTTATTTAGAGCTTTCATTCGA	ACATGTCGAAACAATGGAAAAGA	32.0	34.8	56.6	57.2	213 bp	1.0	42.6
ycf1_p3	TGGATCCTTTATTTAGAGCTTTCA	ACATGTCGAAACAATGGAAAAGAA	33.3	33.3	55.8	57.9	217 bp	1.0	42.6
ycf1_p4	TGGATCCTTTATTTAGAGCTTTCA	ACATGTCGAAACAATGGAAAAG	33.3	36.4	55.8	55.8	217 bp	1.0	42.6
ycf1_p5	TGGATCCTTTATTTAGAGCTTTCA	ACATGTCGAAACAATGGAAA	33.3	35.0	55.8	53.7	217 bp	1.0	42.6