Hotspot

matK

LSC · 2193-3710 · 1518 bp

Back to Corymbia

Pi 0.00246

MarkerSeek score 52.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1518
variable_sites: 10
pi: 0.00246
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TTCCATGATCTCATAGCCAA	CCTTGTTTTGACTGTATCGCA	40.0	42.9	53.8	56.8	1628-1631 bp	1.0	40.6
matK_p2	TTTCCATGATCTCATAGCCA	CCTTGTTTTGACTGTATCGCA	40.0	42.9	53.8	56.8	1629-1632 bp	1.0	40.6
matK_p3	TTCCATGATCTCATAGCCAA	ACCTTGTTTTGACTGTATCGC	40.0	42.9	53.8	56.8	1629-1632 bp	1.0	40.6
matK_p4	TTTCCATGATCTCATAGCCA	ACCTTGTTTTGACTGTATCGC	40.0	42.9	53.8	56.8	1630-1633 bp	1.0	40.6
matK_p5	TTCCATGATCTCATAGCCAA	CCTTGTTTTGACTGTATCGCAC	40.0	45.5	53.8	58.2	1628-1631 bp	1.0	40.6