Hotspot

rps19-rpl2

IRb · 88485-88540 · 56 bp

Back to Corymbia

Pi 0.0155

MarkerSeek score 51.40

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 56
variable_sites: 2
pi: 0.0155
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TCCGCCTTTGTGTTAAGCTT	TTGATTCTTCGTCGCCGTAG	45.0	50.0	58.0	58.1	163 bp	1.0	80.0
rps19-rpl2_p2	TTCCGCCTTTGTGTTAAGCT	TTGATTCTTCGTCGCCGTAG	45.0	50.0	58.0	58.1	164 bp	1.0	80.0
rps19-rpl2_p3	TCCGCCTTTGTGTTAAGCTT	TTGGGGTTATCCTGCACTTG	45.0	50.0	58.0	57.8	221 bp	1.0	78.8
rps19-rpl2_p4	TTCCGCCTTTGTGTTAAGCT	TTGGGGTTATCCTGCACTTG	45.0	50.0	58.0	57.8	222 bp	1.0	78.8
rps19-rpl2_p5	TCCGCCTTTGTGTTAAGCTT	ATTTGATTCTTCGTCGCCGT	45.0	45.0	58.0	58.3	165 bp	1.0	78.6