Hotspot

accD-psaI

LSC · 62386-63065 · 680 bp

Back to Corymbia

Pi 0.00196

MarkerSeek score 54.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 4
pi: 0.00196
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAATCGTACCACGTAATCC	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.6	58.0	831-839 bp	1.0	68.7
accD-psaI_p2	CCAATCGTACCACGTAATCCT	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.6	58.0	830-838 bp	1.0	68.7
accD-psaI_p3	TCCAATCGTACCACGTAATCC	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.6	57.9	837-845 bp	1.0	68.1
accD-psaI_p4	CCAATCGTACCACGTAATCCT	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.6	57.9	836-844 bp	1.0	68.1
accD-psaI_p5	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	806-814 bp	1.0	66.7