Hotspot

atpH-atpI

LSC · 14908-16034 · 1127 bp

Back to Corymbia

Pi 0.00104

MarkerSeek score 53.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1127
variable_sites: 3
pi: 0.00104
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1183-1206 bp	1.0	78.6
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	1239-1262 bp	1.0	78.0
atpH-atpI_p3	AATAGAAGCAAGCCCCACAG	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	1217-1240 bp	1.0	77.8
atpH-atpI_p4	CAATAGAAGCAAGCCCCACA	CGAATCCATGGAGGGTCATC	50.0	55.0	58.2	57.8	1218-1241 bp	1.0	77.6
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1184-1207 bp	1.0	77.5