Hotspot

ycf1

SSC · 129300-135038 · 5739 bp

Back to Corylus

Pi 0.003
MarkerSeek score 41.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5739
variable_sites
63
pi
0.003
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CGTTGGCCCATTTTTGTCAT AGTTTGCAGGAAAATCAACATCA 45.0 34.8 57.5 57.5 266 bp 1.0 49.9
ycf1_p2 TTCGTTGGCCCATTTTTGTC AGTTTGCAGGAAAATCAACATCA 45.0 34.8 57.5 57.5 268 bp 1.0 49.4
ycf1_p3 TCGTTGGCCCATTTTTGTCA AGTTTGCAGGAAAATCAACATCA 45.0 34.8 58.6 57.5 267 bp 1.0 48.8
ycf1_p4 CGTTGGCCCATTTTTGTCAT GTTTGCAGGAAAATCAACATCA 45.0 36.4 57.5 56.1 265 bp 1.0 46.7
ycf1_p5 TTCGTTGGCCCATTTTTGTC GTTTGCAGGAAAATCAACATCA 45.0 36.4 57.5 56.1 267 bp 1.0 46.2