Hotspot

ndhA

SSC · 125132-127421 · 2290 bp

Back to Corylus

Pi 0.00199

MarkerSeek score 48.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2290
variable_sites: 22
pi: 0.00199
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCCCAGTTACCATAGGG	CCCGCCGGGTTTTATCAATT	55.0	50.0	58.1	58.9	2492-2528 bp	1.0	73.4
ndhA_p2	TGACCCCAGTTACCATAGGG	CCGCCGGGTTTTATCAATTT	55.0	45.0	58.1	57.0	2491-2527 bp	1.0	73.0
ndhA_p3	GACCCCAGTTACCATAGGGA	CCCGCCGGGTTTTATCAATT	55.0	50.0	57.8	58.9	2491-2527 bp	1.0	72.8
ndhA_p4	GACCCCAGTTACCATAGGGA	CCGCCGGGTTTTATCAATTT	55.0	45.0	57.8	57.0	2490-2526 bp	1.0	72.4
ndhA_p5	TGACCCCAGTTACCATAGGG	GCCGGGTTTTATCAATTTGCA	55.0	42.9	58.1	58.0	2489-2525 bp	1.0	72.3