Hotspot

ndhF-rpl32

SSC · 117318-118213 · 896 bp

Back to Corylus

Pi 0.00674
MarkerSeek score 48.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
896
variable_sites
23
pi
0.00674
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CACATGCGGCGAAGATTTTT TTTGATATAGAAGTGCGTTTTT 45.0 27.3 58.0 52.4 830-1067 bp 1.0 45.8
ndhF-rpl32_p2 CACATGCGGCGAAGATTTTT TTTTGATATAGAAGTGCGTTTT 45.0 27.3 58.0 52.4 831-1068 bp 1.0 45.8
ndhF-rpl32_p3 CACATGCGGCGAAGATTTTT TTTTTGATATAGAAGTGCGTTT 45.0 27.3 58.0 52.4 832-1069 bp 1.0 45.8
ndhF-rpl32_p4 CACATGCGGCGAAGATTTTT TTTTGATATAGAAGTGCGTTTTT 45.0 26.1 58.0 53.3 831-1068 bp 1.0 45.8
ndhF-rpl32_p5 CACATGCGGCGAAGATTTTT TTTTTGATATAGAAGTGCGTTTT 45.0 26.1 58.0 53.3 832-1069 bp 1.0 45.8