Hotspot

trnT-GGT-psbD

LSC · 35471-36877 · 1407 bp

Back to Corylus

Pi 0.00277
MarkerSeek score 58.40
Primer available yes
Label trnT-GGT-psbD

Feature Metadata

label_name
trnT-GGT-psbD
feature_type
igs
region
LSC
length_bp
1407
variable_sites
15
pi
0.00277
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGT-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1444-1587 bp 1.0 82.3
trnT-GGT-psbD_p2 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1458-1601 bp 1.0 82.2
trnT-GGT-psbD_p3 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1447-1590 bp 1.0 82.1
trnT-GGT-psbD_p4 TCAGTGGTAGAGTAACGCCA GACCGGACCAACCTACAAAA 50.0 50.0 58.1 57.7 1461-1604 bp 1.0 82.0
trnT-GGT-psbD_p5 GTGGTAGAGTAACGCCATGG TAGACCGGACCAACCTACAA 55.0 50.0 58.1 57.7 1460-1603 bp 1.0 81.8