Hotspot

trnS-GCT-trnG-UCC

LSC · 9565-10460 · 896 bp

Back to Corylus

Pi 0.0078
MarkerSeek score 53.60
Primer available yes
Label trnS-GCT-trnG-UCC

Feature Metadata

label_name
trnS-GCT-trnG-UCC
feature_type
igs
region
LSC
length_bp
896
variable_sites
23
pi
0.0078
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCT-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GGACCCCTTAACTATTACGGG 50.0 52.4 58.0 57.2 957-1008 bp 1.0 71.8
trnS-GCT-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA GGGACCCCTTAACTATTACGG 50.0 52.4 58.0 57.2 958-1009 bp 1.0 71.8
trnS-GCT-trnG-UCC_p3 AATCCGACGCTTTAGTCCAC GGACCCCTTAACTATTACGGG 50.0 52.4 58.0 57.2 956-1007 bp 1.0 71.8
trnS-GCT-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC GGGACCCCTTAACTATTACGG 50.0 52.4 58.0 57.2 957-1008 bp 1.0 71.7
trnS-GCT-trnG-UCC_p5 ACAAAAGCGGAAAGAGAGGG GGACCCCTTAACTATTACGGG 50.0 52.4 58.1 57.2 1017-1068 bp 1.0 70.9