Hotspot

rps16-trnQ-TTG

LSC · 6670-8080 · 1411 bp

Back to Corylus

Pi 0.00358

MarkerSeek score 54.30

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 1411
variable_sites: 16
pi: 0.00358
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	TCCACAACCGACCATATCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1434-1513 bp	1.0	83.3
rps16-trnQ-TTG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1412-1491 bp	1.0	83.2
rps16-trnQ-TTG_p3	TCCACAACCGACCATATCCT	GGGTTTTGGTCCCGGTATTC	50.0	55.0	58.1	58.5	1455-1534 bp	1.0	79.6
rps16-trnQ-TTG_p4	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	1433-1512 bp	1.0	79.5
rps16-trnQ-TTG_p5	CCACAACCGACCATATCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.3	58.1	1433-1512 bp	1.0	78.6