Hotspot

ycf1

SSC · 128126-133690 · 5565 bp

Back to Corylopsis

Pi 0.00231

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5565
variable_sites: 72
pi: 0.00231
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGACAAGCTAGGTGGATACG	GGATGAATTCCACTTTCACT	50.0	40.0	56.8	53.2	244 bp	1.0	41.6
ycf1_p2	GACAAGCTAGGTGGATACGT	GGATGAATTCCACTTTCACT	50.0	40.0	56.7	53.2	243 bp	1.0	41.6
ycf1_p3	TGACAAGCTAGGTGGATACG	GGATGAATTCCACTTTCACTT	50.0	38.1	56.8	54.1	244 bp	1.0	41.6
ycf1_p4	GACAAGCTAGGTGGATACGT	GGATGAATTCCACTTTCACTT	50.0	38.1	56.7	54.1	243 bp	1.0	41.6
ycf1_p5	TGACAAGCTAGGTGGATACG	GGATGAATTCCACTTTCACTTT	50.0	36.4	56.8	54.9	244 bp	1.0	41.6