Hotspot

rps18

LSC · 71467-71772 · 306 bp

Back to Corylopsis

Pi 0.0014
MarkerSeek score 29.30
Primer available yes
Label rps18

Feature Metadata

label_name
rps18
feature_type
gene
region
LSC
length_bp
306
variable_sites
3
pi
0.0014
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
29.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18_p1 TGTGATTGTGATTTGACCGA ACATCAATCGGCGTTTGAGT 40.0 45.0 55.3 58.2 414 bp 0.928571 54.2
rps18_p2 TGTGATTGTGATTTGACCGAA ACATCAATCGGCGTTTGAGT 38.1 45.0 56.1 58.2 414 bp 0.928571 52.6
rps18_p3 TTGTGATTGTGATTTGACCGA ACATCAATCGGCGTTTGAGT 38.1 45.0 56.1 58.2 415 bp 0.928571 52.6
rps18_p4 TGTGATTGTGATTTGACCGAAA ACATCAATCGGCGTTTGAGT 36.4 45.0 56.8 58.2 414 bp 0.928571 50.4
rps18_p5 TTGTGATTGTGATTTGACCGAA ACATCAATCGGCGTTTGAGT 36.4 45.0 56.8 58.2 415 bp 0.928571 50.4