Hotspot

rpoA

LSC · 81355-82368 · 1014 bp

Back to Corylopsis

Pi 0.00279

MarkerSeek score 33.40

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 15
pi: 0.00279
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CATTTCGTCGTCCAGTAGCA	CCGGTACTTGTGGATTCAGG	50.0	55.0	58.0	58.0	1632-1641 bp	1.0	81.6
rpoA_p2	CATTTCGTCGTCCAGTAGCA	CGGTACTTGTGGATTCAGGG	50.0	55.0	58.0	58.0	1631-1640 bp	1.0	81.6
rpoA_p3	CATTTCGTCGTCCAGTAGCA	GATAAAAGGCCCTGGTCTCG	50.0	55.0	58.0	58.0	1517-1526 bp	1.0	81.5
rpoA_p4	CATTTCGTCGTCCAGTAGCA	TACAAGAAGAGGGACACCGT	50.0	50.0	58.0	58.0	1610-1619 bp	0.928571	77.9
rpoA_p5	CCTTCGGGGGATGGATCTAT	TACAAGAAGAGGGACACCGT	55.0	50.0	58.0	58.0	2009-2018 bp	0.928571	77.5