Hotspot

ndhA

SSC · 124117-126277 · 2161 bp

Back to Corylopsis

Pi 0.00293

MarkerSeek score 53.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2161
variable_sites: 34
pi: 0.00293
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	47.6	50.0	58.1	58.5	2371-2386 bp	1.0	69.1
ndhA_p2	TGAACCCAGTTACCATAGGGA	GCCCACCGGGTTTTATCAAT	47.6	50.0	57.8	58.5	2374-2389 bp	1.0	68.1
ndhA_p3	ACCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAA	47.6	52.6	58.1	58.0	2371-2386 bp	1.0	65.0
ndhA_p4	TGAACCCAGTTACCATAGGGA	GCCCACCGGGTTTTATCAA	47.6	52.6	57.8	58.0	2374-2389 bp	1.0	63.9
ndhA_p5	CCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	50.0	50.0	56.2	58.5	2370-2385 bp	1.0	63.4