Hotspot

rpl32-trnL-UAG

SSC · 117460-118506 · 1047 bp

Back to Corylopsis

Pi 0.0034
MarkerSeek score 63.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1047
variable_sites
18
pi
0.0034
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCGTTAAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 1161-1174 bp 1.0 82.1
rpl32-trnL-UAG_p2 GGGCAGCGTTAAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 1162-1175 bp 1.0 82.1
rpl32-trnL-UAG_p3 GGGCAGCGTTAAAAGCTTTT TTTAGAAGATCCCATGCCGC 45.0 50.0 57.9 58.0 1226-1239 bp 1.0 81.7
rpl32-trnL-UAG_p4 AAAAGGAAGGGGTATTGGGC AGCGTGTCTACCGATTTCAC 50.0 50.0 57.8 58.0 1177-1190 bp 1.0 81.3
rpl32-trnL-UAG_p5 AAAAGGAAGGGGTATTGGGC CAGCGTGTCTACCGATTTCA 50.0 50.0 57.8 58.0 1178-1191 bp 1.0 81.2